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	Provedor de dados ArchiMer (5) Autor Boulo, Viviane (5) Mialhe, Eric (5) Paolucci, Francis (5) Clavies, Christine (2) Grizel, Henri (2) Mourton, Chantal (2) Pau, Bernard (2) Rogier, Hervé (2) Bachere, Evelyne (1) Chagot, Dominique (1) Elston, Ralph (1) Hervaud, Eliane (1) Hervaud, Eric (1) Hervio, Dominique (1) Le Gall, Guillaume (1) Le Gall, Gyslaine (1) Noel, Danielle (1) Pau, B (1) Mais... Palavra-chave Bonamia ostreae (1) Diagnosis (1) Molluscs (1) Monoclona antibody (1) Monoclonal antibodies (1) Monoclonal antibody technology (1) Ostrea edulis (1) Oyster (1) Pathology (1) Pecten maximus (1) Rickettsiales like organism (1) Mais... Tipo do documento Text (5) Ano 1992 (2) 1991 (2) 1988 (1) País France (5) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Monoclonal antibodies : a tool for molluscan pathology ArchiMer Mialhe, Eric; Boulo, Viviane; Grizel, Henri; Rogier, Hervé; Paolucci, Francis. Hybridoma technology is reviewed, and the characteristics of monoclonal antibodies are compared with those of polyclonal antibodies. The contribution of monoclonal antibodies to molluscan pathology is developed with special emphasis on their use as diagnostic tools. The results of studies with monoclonal antibodies prepared against the protozoan oyster pathogen Bonamia ostreae are briefly described. Tipo: Text Palavras-chave: Pathology; Molluscs; Monoclonal antibody technology. Ano: 1988 URL: http://archimer.ifremer.fr/doc/1988/publication-3121.pdf Monoclonal antibodies against Bonamia ostreae (Protozoa: Ascetospera), an intrahaemocytic parasite of flat oyster Ostrea edulis (Mollusca : Bivlavia) ArchiMer Rogier, Hervé; Hervio, Dominique; Boulo, Viviane; Clavies, Christine; Hervaud, Eliane; Bachere, Evelyne; Mialhe, Eric; Grizel, Henri; Pau, Bernard; Paolucci, Francis. The protozoan Bonamia ostreae (Ascetospora), a paasite of the flat oyster Ostrea edulis, was purified by differential and isopycnic centrifugations. Mice of the strain Balb/c were immunized with purified parasites and hybridomas were prepared by fusion of immunized mouse splenocytes with the mouse myeloma cell line P3-X63-Ag8-653. From the fusion, 12 clones were isolated and saved. The resulting antibodies were characterized with a solid phase radioimmunoassay (RIA). Antibodies from 7 clones reacted only with B. ostreae and not with normal host tissue. Although some of the antibiodies identified. One of these antibodies (20B2-1B12) has an apparent association constant of ca 3 X 10(8) M-1 and bound to a number of different sites on the parasite. These... Tipo: Text Palavras-chave: Monoclonal antibodies; Oyster; Ostrea edulis; Bonamia ostreae. Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2756.pdf Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maximus (Bivalvia): application to indirect immunofluorescence diagnosis ArchiMer Le Gall, Guillaume; Mourton, Chantal; Boulo, Viviane; Paolucci, Francis; Pau, B; Mialhe, Eric. Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maximus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis. Tipo: Text Ano: 1992 URL: http://archimer.ifremer.fr/doc/00190/30135/28778.pdf Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maxiumus (Bivalvia): application to indirect immunofluorescence diagnosis ArchiMer Le Gall, Gyslaine; Mourton, Chantal; Boulo, Viviane; Paolucci, Francis; Pau, Bernard; Mialhe, Eric. Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maxiumus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis Tipo: Text Palavras-chave: Diagnosis; Rickettsiales like organism; Monoclona antibody; Pecten maximus. Ano: 1992 URL: http://archimer.ifremer.fr/doc/1992/publication-2762.pdf Preparation and characterization of monoclonal antibodies against neoplastic hemocytes of Mytilus edulis (Bivalvia) ArchiMer Noel, Danielle; Boulo, Viviane; Chagot, Dominique; Mialhe, Eric; Paolucci, Francis; Clavies, Christine; Hervaud, Eric; Elston, Ralph. Mytilus edulis develop progressive and fatal hemocytic neoplasia. We have generated monoclonal antibodies using neoplastic hemocytes from highly infected individuals collected from Puget Sound in Washington State (USA). Twenty-seven monoclonal antibodies against neoplatic cell antigens were obtained. Specificity of 2 purified antibodies, 14F1 and 16G10, was demonstrated by immunofluorescence when incubated with neoplastic hemocytes, whereas these anbidodies did not react with normal cells. This specificity was confirmed by immunogold labelling and electron microscopic visualization. The technique permitted localization of the epitopes recognized by the monoclonal antibodies on the plasma membrane. These monoclonal antibodies are suitable reagents for... Tipo: Text Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/publication-2757.pdf Registros recuperados: 5 Primeira ... 1 ... Última

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